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Rapid Freezing using Sandwich Freezing Device for Good Ultrastructural Preservation of Biological Specimens in Electron Microscopy
JoVE Journal
Biologia
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JoVE Journal Biologia
Rapid Freezing using Sandwich Freezing Device for Good Ultrastructural Preservation of Biological Specimens in Electron Microscopy

Rapid Freezing using Sandwich Freezing Device for Good Ultrastructural Preservation of Biological Specimens in Electron Microscopy

DOI:
10.3791/62431-v

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09:03 min

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July 19, 2021

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, , , , ,
1Medical Mycology Research Center,Chiba University, 2Marine Works Japan Ltd.

Capítulos

  • 00:04Introduction
  • 00:48Rapid Freezing of Cell Suspensions for Freeze-Substitution
  • 07:19Results: Ultrastructural Preservation of Biological Specimens in Electron Microscopy Using Sandwich Freezing Device
  • 08:03Conclusion

Summary

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Here we show how to use the sandwich freezing device for rapid freezing of biological specimens, including bacteria, yeast, cultured cells, isolated cells, animal and human tissues, and viruses. We also show how to prepare specimens for ultrathin sectioning after rapid freezing.

Tags

Keywords: Rapid FreezingSandwich Freezing DeviceUltrastructural PreservationBiological SpecimensElectron MicroscopyCell FixationIce Crystal FormationCopper DiscsGlow DischargeCentrifugationPropane FreezingGlutaraldehyde FixationFreeze-substitution

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