/

Imunologia e Infecção

/

使用共聚焦显微镜研究 利什曼原虫 的吞噬作用

JoVE Journal
Imunologia e Infecção
Author Produced

É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.

JoVE Journal Imunologia e Infecção

Investigating the Phagocytosis of Leishmania using Confocal Microscopy

Please note that all translations are automatically generated. Click here for the English version.

使用共聚焦显微镜研究利什曼原虫的吞噬作用

DOI:

10.3791/62459-v

•

08:41 min

•

July 29, 2021

•

Amanda R. Paixão*¹, Beatriz R. S. Dias*¹, Luana C. Palma, Natália M. Tavares, Cláudia I. Brodskyn, Juliana P. B de Menezes, Patricia S. T. Veras²

¹Laboratory of Host-Parasite Interaction and Epidemiology,Gonçalo Moniz Institute, ²National Institute of Science and Technology of Tropical Diseases – National Council for Scientific Research and Development (CNPq)

Capítulos

00:05Introduction
01:20Leishmania Phagocytosis Assessment by Macrophages
02:50Evaluation of Leishmania‐Induced Vacuole Maturation
04:55Confocal Microscopy Acquisition and Quantification
06:08Results: Assessment of Leishmania spp. Phagocytosis
07:43Conclusion

Summary

Tadução automática

利 什曼原虫 感染中与吞噬作用相关的机制仍然知之甚少。在这里，我们描述了评估 利什曼原虫 与宿主细胞相互作用期间发生的早期事件的方法。

Tags

Keywords: Phagocytosis Leishmania Confocal Microscopy THP-1 Cells Macrophages Promastigotes PV Biogenesis Transfection

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

在Live主机转利什曼原虫寄生虫在体内成像

可视化的细胞与细胞转移的艾滋病毒，艾滋病毒和现场共聚焦显微镜的荧光克隆

使用双光子显微镜在受感染的肺部的嗜中性粒细胞吞噬和NET形成的直接观察

活体显微镜的脾：寄生虫的流动性和血流量的定量分析

活细胞视频显微镜病病原菌的吞噬作用

使用一个调查益生素对肺炎球菌定植的影响<em>体外</em>粘附试验

评估隔离肺泡巨噬细胞的激光共聚焦显微镜抗真菌活性

肝脏活体如下显微成像<em>利什曼原虫</em>感染：肝脏血流动力学的评估

一种用于丝状真菌菌落细菌生物膜的定性多尺度分析的新方法利用共聚焦和电子显微镜

利用共焦显微镜可视化巨噬细胞胞外陷阱

Read Article