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Laser micro-bestråling til undersøgelse DNA rekruttering i S fase
JoVE Journal
Biologia
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JoVE Journal Biologia
Laser Micro-Irradiation to Study DNA Recruitment During S Phase

Laser micro-bestråling til undersøgelse DNA rekruttering i S fase

DOI:
10.3791/62466-v

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07:11 min

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April 16, 2021

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1Department of Biochemistry and Molecular Pharmacology,New York University School of Medicine, 2Laura and Isaac Perlmutter Cancer Center,New York University School of Medicine, 3Howard Hughes Medical Institute,New York University School of Medicine

Capítulos

  • 00:04Introduction
  • 00:47Preparation of Cells for Micro-Irradiation
  • 01:18Selecting S Phase Cells for Imaging
  • 02:40Micro-Irradiation for Immunofluorescence Staining or Time Lapse Imaging
  • 03:44Time Lapse Imaging
  • 04:47Results: DNA Recruitment During S Phase After Micro-Irradiation
  • 06:23Conclusion

Summary

Tadução automática

Tadução automática

Denne protokol beskriver en ikke-invasiv metode til effektivt at identificere S-fase celler til downstream mikroskopi undersøgelser, såsom måling AF DNA reparation protein rekruttering ved laser mikro-bestråling.

Tags

Laser Micro-irradiationDNA RepairS PhasePCNASpatiotemporal ResolutionDNA Damage Repair Protein KineticsConfocal ImagingOlaparibEnvironmental ChamberMicroscopeMPlumBinary LinesROIND StimulationTime Schedule

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