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通过Ussing Chamber技术评估天然组织中肠道紧密连接屏障和离子渗透性的功能评估
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Biologia
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JoVE Journal Biologia
Functional Assessment of Intestinal Tight Junction Barrier and Ion Permeability in Native Tissue by Ussing Chamber Technique
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通过Ussing Chamber技术评估天然组织中肠道紧密连接屏障和离子渗透性的功能评估

DOI:
10.3791/62468-v

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06:43 min

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May 26, 2021

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, ,
1Laboratory of Physiology, Graduate School of Nutritional and Environmental Sciences,University of Shizuoka

Capítulos

  • 00:04Introduction
  • 00:55Stripping the Muscle Layer and Preparing the Intestinal Sheet
  • 02:12Mounting Intestinal Preparations in Ussing Chambers
  • 03:05Measurement of Dilution Potential (Open Circuit Conditions)
  • 04:07Transepithelial Electrical Conductance and Baseline Isc (Short-Circuit Conditions)
  • 04:55Results: Transepithelial Electrical Conductance and Dilution Potential in Cldn15-/- Mice
  • 06:12Conclusion

Summary

Tadução automática

Tadução automática

肠上皮不仅提供营养吸收，而且保护免受有毒物质的侵害。顶端最顶端的上皮细胞间连接，即紧密连接，调节细胞旁溶质和离子通透性。这里描述了使用Ussing室技术制备粘膜片和评估紧密连接的离子选择性的方案。

Tags

Ussing ChamberTight JunctionIntestinal PermeabilityIon TransportFunctional AssessmentClaudin-15 KnockoutMuscle Layer DissectionHEPES Buffer

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