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구강에서 공생 사카리박테리아의 안정적인 이진 문화 구축
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Establishing Stable Binary Cultures of Symbiotic Saccharibacteria from the Oral Cavity
DOI:

07:32 min

April 13, 2021

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Capítulos

  • 00:04Introduction
  • 01:11Preparation Initiating Host Cultures and Sterilizing Equipment
  • 01:55Obtaining Sample of Oral Bacteria
  • 02:30Preparation of Filtrate from Oral Sample
  • 03:28Concentration of the Saccharibacteria Cells by Centrifugation
  • 04:02Infection of Host Cultures with Saccharibacteria-enriched Filtrate
  • 04:47Confirmation of Infection by PCR and Verification of Purity
  • 05:36Results: Confirmation of Infection and Purity of Binary Cultures of Symbiotic Saccharibacteria
  • 06:54Conclusion

Summary

Tadução automática

우리는 치과 플라크를 필터링하고 숙주 박테리아와 공동 배양하여 새로운 세균 성 물리, 사카리박테리아의 성장하기 어려운 구성원을 격리하는 방법을 보여줍니다.

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