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The Automated Crystallography Pipelines at the EMBL HTX Facility in Grenoble
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Bioquímica
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JoVE Journal Bioquímica
The Automated Crystallography Pipelines at the EMBL HTX Facility in Grenoble

The Automated Crystallography Pipelines at the EMBL HTX Facility in Grenoble

DOI:
10.3791/62491-v

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06:50 min

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June 05, 2021

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, , , , , , , , , ,
1European Molecular Biology Laboratory, 2ALPX S.A.S., 3Institut de Biologie Structurale

Capítulos

  • 00:04Introduction
  • 00:32Crystallization Screening Experiments
  • 02:42Crystal Optimization Experiments
  • 03:18Crystal Harvesting and Tracking
  • 04:21Synchrotron X-Ray Diffraction Experiments
  • 05:27Resultados
  • 06:08Conclusion

Summary

Tadução automática

Tadução automática

Here, we describe how to use the automated macromolecular crystallography pipelines for protein-to-structure, rapid ligand-protein complex analysis and large-scale fragment screening based on the CrystalDirect technology at the HTX Laboratory in EMBL Grenoble.

Tags

Automated CrystallographyEMBL HTX FacilityGrenobleCrystalDirect TechnologyCRIM SoftwareCrystal Graphic Information Management SystemCrystallization ProtocolCrystallization ScreensHigh Throughput Crystallization LabAutomated ImagersCRIMSCrystallization PlatesCrystallization ExperimentsCrystal HarvestingProtein Structure DeterminationFragment ScreeningLigand Screening

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