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小鼠巨核细胞祖细胞的分离

JoVE Journal
Biologia

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JoVE Journal Biologia

Isolation of Mouse Megakaryocyte Progenitors

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小鼠巨核细胞祖细胞的分离

DOI:

10.3791/62498-v

•

10:30 min

•

May 20, 2021

•

Quentin Kimmerlin, Manuela Tavian, Christian Gachet, François Lanza, Nathalie Brouard

¹Université de Strasbourg, INSERM UMR S1255, EFS Grand-EST, ²UMR-S1113 –IRFAC, Université de Strasbourg

Capítulos

00:04Introduction
00:38Mouse Bone Collection
03:10Magnetic Depletion of Lineage Positive Cells
07:17Results: Purification of MouseMEP and MKp from Femurs, Tibias, and Pelvic Bones by Flow Cytometry
09:50Conclusion

Summary

Tadução automática

该方法描述了通过流式细胞术从小鼠股骨，胫骨和盆骨中纯化MEP和MKp。

Tags

Keywords: Mouse Megakaryocyte Progenitors Cell Sorting MEP MKP Bone C57 Black Six Iliac Crest Femur Tibia PBS NBCS Dissection Cell Isolation

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