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A High-Throughput Enzyme-Coupled Activity Assay to Probe Small Molecule Interaction with the dNTPase SAMHD1
This content is Free Access.
Capítulos
- 00:00Introduction
- 00:25Preparation of Complete Assay Buffers
- 01:08SAMHD1 Inhibition Assay
- 03:45SAMHD1 Activator and Substrate Screen
- 05:57Results: Enzyme-Coupled Activity Assay to Screen SAMHD1 Activators or Inhibitors
- 07:49Conclusion
SAMHD1 is a deoxynucleoside triphosphate triphosphohydrolase with critical roles in human health and disease. Here we present a versatile enzyme-coupled SAMHD1 activity assay, deployed in a 384-well microplate format, that allows for the evaluation of small molecules and nucleotide analogues as SAMHD1 substrates, activators, and inhibitors.
