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Megakaryocyte Culture in 3D Methylcellulose-Based Hydrogel to Improve Cell Maturation and Study the Impact of Stiffness and Confinement
JoVE Journal
Biologia
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JoVE Journal Biologia
Megakaryocyte Culture in 3D Methylcellulose-Based Hydrogel to Improve Cell Maturation and Study the Impact of Stiffness and Confinement
DOI:

07:53 min

August 26, 2021

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Capítulos

  • 00:05Introduction
  • 00:53Cell Embedding in Methylcellulose Hydrogel
  • 03:03Cell Resuspension for Proplatelet Analysis
  • 04:24Cell Fixation and Retrieval for Future Analyses
  • 05:16Results: Megakaryocyte Characteristics and Proplatelet Quantification According to Culture Conditions
  • 07:08Conclusion

Summary

Tadução automática

It is now acknowledged that the three-dimensional environment of cells can play an important role in their behavior, maturation and/or differentiation. This protocol describes a three-dimensional cell culture model designed to study the impact of physical containment and mechanical constraints on megakaryocytes.

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