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Analysis of Lymph Node Volume by Ultra-High-Frequency Ultrasound Imaging in the Braf/Pten Genetically Engineered Mouse Model of Melanoma
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Analysis of Lymph Node Volume by Ultra-High-Frequency Ultrasound Imaging in the Braf/Pten Genetically Engineered Mouse Model of Melanoma

Analysis of Lymph Node Volume by Ultra-High-Frequency Ultrasound Imaging in the Braf/Pten Genetically Engineered Mouse Model of Melanoma

DOI:
10.3791/62527-v

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08:18 min

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September 08, 2021

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, , ,
1Institute of Clinical Physiology,National Research Council (IFC-CNR), 2Oncogenomics Unit,Core Research Laboratory, ISPRO

Capítulos

  • 00:04Introduction
  • 01:04Melanoma Induction
  • 01:58Imaging Procedure
  • 04:18Post-Processing of Ultrasound Images
  • 05:55Tissue-Specific and Inducible Braf/Pten Metastatic Melanoma Mouse Model
  • 07:29Conclusion

Summary

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Tadução automática

Melanoma is a very aggressive disease that quickly spreads to other organs. This protocol describes the application of ultra-high-frequency ultrasound imaging, coupled with 3D rendering, to monitor the volume of the inguinal lymph nodes in the Braf/Pten mouse model of metastatic melanoma.

Tags

Keywords: Lymph Node VolumeUltra-high-frequency Ultrasound ImagingBraf/Pten Genetically Engineered Mouse ModelMelanomaMetastatic DiseasePreclinical Mouse Models4-hydroxytamoxifenPrimary TumorSubcutaneous MetastasesInguinal Lymph NodesHistological StudiesAnesthesiaPhysiological ParametersDepilatory AgentAcoustic Coupling MediumLinear ProbeRegion-of-interestScan DistanceStep Size

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