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在微流体平台内开发 3D 组织人类心脏组织

JoVE Journal
Bioengenharia
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JoVE Journal Bioengenharia

Developing 3D Organized Human Cardiac Tissue within a Microfluidic Platform

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在微流体平台内开发 3D 组织人类心脏组织

DOI:

10.3791/62539-v

•

10:42 min

•

June 15, 2021

•

Jaimeson Veldhuizen, Mehdi Nikkhah²

¹School of Biological and Health Systems Engineering,Arizona State University, ²Biodesign Virginia G. Piper Center for Personalized Diagnostics,Arizona State University

Capítulos

00:00Introduction
01:05hCF Dissociation
03:26CM Dissociation
05:59Collagen Preparation
06:59Hydrogel Mixture and Cell Preparation
07:41Device Insertion
09:32Results: Cardiac Tissue Formation
10:13Conclusion

Summary

Tadução automática

本协议的目的是解释和演示高度对齐的人类心脏组织的三维（3D）微流体模型的发展，该模型由干细胞衍生心肌细胞组成，与心脏成纤维细胞（CFs）在生物仿真、胶原蛋白基水凝胶中共同培养，用于心脏组织工程、药物筛查和疾病建模。

Tags

Microfluidic Platform Cardiovascular Disease Myocardium Stem Cell-derived Cardiomyocytes Cardiac Fibroblasts Cell Dissociation Cell Culture Cell Alignment

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