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Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction
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Bioquímica
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JoVE Journal Bioquímica
Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction

Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction

DOI:
10.3791/62547-v

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09:19 min

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June 01, 2022

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, , , , , ,
1Department of Molecular Biology and Biochemistry,University of California Irvine, 2Department of Ecology and Evolutionary Biology,University of California Irvine, 3Entech Instruments Inc.

Capítulos

  • 00:04Introduction
  • 00:59Stable Isotope Probing in Biological Samples Preparation
  • 02:17Sample Extraction
  • 04:10Analyze Samples on the Gas Chromatography Mass Spectrometer (GC-MS)
  • 04:56Data Analysis
  • 05:45Results: GC-MS, Heatmaps, and Data Analysis of the 13C-Labeled Volatiles in Fecal, Saliva, Sewage, Cultured and Uncultured Sputum Samples
  • 08:24Conclusion

Summary

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This protocol describes the extraction of volatile organic compounds from a biological sample with the vacuum-assisted sorbent extraction method, gas chromatography coupled with mass spectrometry using the Entech Sample Preparation Rail, and data analysis. It also describes culture of biological samples and stable isotope probing.

Tags

Keywords: Microbial Volatile Organic CompoundsVASEVacuum-assisted Sorbent ExtractionVolatile MetabolitesBiomarker DiscoveryFecal SamplesSewage SamplesSaliva SamplesSputum SamplesVOA VialsCold Plate13C GlucoseDeuteriumBHI MediumTechnical Triplicates

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