Journal
/
Bioengenharia
/
Modulating Shape of Polyester Based Polymersomes using Osmotic Pressure
JoVE Journal
Bioengenharia
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Bioengenharia
Modulating Shape of Polyester Based Polymersomes using Osmotic Pressure

Modulating Shape of Polyester Based Polymersomes using Osmotic Pressure

DOI:
10.3791/62548-v

•

06:01 min

•

April 21, 2021

•

, ,
1Department of Chemical and Biomolecular Engineering,Clemson University, 2Department of Biological Sciences,Clemson University, 3Department of Bioengineering,Clemson University

Capítulos

  • 00:05Introduction
  • 00:45Spherical Polymersome Formation Using a Solvent Injection Method
  • 02:24Dialysis to Remove Organic Solvent
  • 03:05Dialysis Against Salt Gradients and Shape Modulated Polymersome Characterization
  • 03:50Results: Modulating Shape of Polyester Based Polymersomes using Osmotic Pressure
  • 05:36Conclusion

Summary

Tadução automática

Tadução automática

Polymersomes are self-assembled polymeric vesicles that are formed in spherical shapes to minimize Gibb's Free Energy. In the case of drug delivery, more elongated structures are beneficial. This protocol establishes methods to create more rod-like polymersomes, with elongated aspect ratios, using salt to induce osmotic pressure and reduce internal vesicle volumes.

Tags

Keywords: PolyesterPolymersomesNanoparticle ShapeOsmotic PressureDrug DeliveryElongated PolymersomesHydrophobic And Hydrophilic DrugsBlood-brain BarrierSolvent InjectionDynamic Light ScatteringDialysis

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/62548?article_type=v

Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor
check_url/pt/62548?article_type=v

Shape Memory Polymers for Active Cell Culture
check_url/pt/62548?article_type=v

Microfluidic Fabrication of Polymeric and Biohybrid Fibers with Predesigned Size and Shape
check_url/pt/62548?article_type=v

Fabrication of a Bioactive, PCL-based “Self-fitting” Shape Memory Polymer Scaffold
check_url/pt/62548?article_type=v

Forming Giant-sized Polymersomes Using Gel-assisted Rehydration
check_url/pt/62548?article_type=v

Ammonia Synthesis at Low Pressure
check_url/pt/62548?article_type=v

Pattern-based Search of Epigenomic Data Using GeNemo
check_url/pt/62548?article_type=v

Light-sheet Fluorescence Microscopy to Capture 4-Dimensional Images of the Effects of Modulating Shear Stress on the Developing Zebrafish Heart
check_url/pt/62548?article_type=v

A Rapid Image-based Bacterial Virulence Assay Using Amoeba
check_url/pt/62548?article_type=v

Mouse Model of Pressure Ulcers After Spinal Cord Injury

Read Article