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体外使用小鼠胚胎干细胞进行血管疾病建模和药物测试的血管生成的三维发芽测定

JoVE Journal
Biologia

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JoVE Journal Biologia

In Vitro Three-Dimensional Sprouting Assay of Angiogenesis Using Mouse Embryonic Stem Cells for Vascular Disease Modeling and Drug Testing

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体外使用小鼠胚胎干细胞进行血管疾病建模和药物测试的血管生成的三维发芽测定

DOI:

10.3791/62554-v

•

08:04 min

•

May 11, 2021

•

Georgios Galaris, Jérémy H. Thalgott, Eliott Teston, Franck P.G. Lebrin^2,3

¹Einthoven Laboratory for Experimental Vascular Medicine, Department of Internal Medicine (Nephrology),Leiden University Medical Center, ²Institute Physics for Medicine Paris,INSERM U1273, ESPCI Paris, CNRS FRE 2031, ³MEMOLIFE Laboratory of Excellence and PSL Research University

Capítulos

00:04Introduction
01:14Embryoid Bodies (EB) Formation in Hanging Drop
03:02Floating EBs Culture for Vascular Differentiation
03:413D Sprouting Angiogenesis Assay and Immunofluorescence Staining
05:44Results: Microscopic Analysis and Quantification of Mouse EBs Undergoing Angiogenesis Assay
07:29Conclusion

Summary

Tadução automática

该测定利用分化成在3D胶原蛋白凝胶中培养的拟胚体的小鼠胚胎干细胞来分析体外控制发芽血管生成的生物学过程。该技术可用于测试药物，模拟疾病以及在胚胎致命的缺失背景下研究特定基因。

Tags

Keywords: In Vitro Sprouting Angiogenesis Mouse Embryonic Stem Cells Vascular Disease Modeling Drug Testing Endothelial Tip Cells Cell Migration Genetic Screening Pluripotency Karyotyping Mesoderm Differentiation Low Attachment Polystyrene Dishes

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