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Selection of Transporter-Targeted Inhibitory Nanobodies by Solid-Supported-Membrane (SSM)-Based Electrophysiology
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Capítulos
- 00:04Introduction
- 00:58Measuring the Solute Transportation: Determination of Saturation Conditions
- 03:16Serial Classification of Inhibitory and Non-Inhibitory Nanobodies
- 05:59IC50 Measurement with Inhibitory Nanobodies
- 07:40Results: Selection of Transporter Targeted Inhibitory Nanobodies by SSM Based Electrophysiology
- 08:41Conclusion
Nanobodies are important tools in structural biology and pose a great potential for the development of therapies. However, the selection of nanobodies with inhibitory properties can be challenging. Here we demonstrate the use of solid-supported-membrane (SSM)-based electrophysiology for the classification of inhibitory and non-inhibitory nanobodies targeting electrogenic membrane transporters.
Tags
Keywords:
Transporter-targeted Inhibitory NanobodiesSolid-supported-membrane (SSM)-based ElectrophysiologyHigh-throughput ScreeningElectrogenic TransportersProteoliposomesMembrane VesiclesNanobodiesMedical ApplicationsElectrogenic TransportersSSM BufferActivating SSM BuffersProteoliposome-coated ChipConductivityCapacitanceActivating SolutionsNonactivating BufferBAB SequenceProtein-free Liposomes
