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1Department of Internal Medicine, Ribeirão Preto Medical School,University of São Paulo, 2Centre for Computational Biology,University of Birmingham, 3Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences,University of Birmingham, 4Cancer Genetics and Evolution Laboratory, Cancer Research UK, Institute of Genetics & Molecular Medicine,The University of Edinburgh, 5Ribeirão Preto School of Physical Education and Sport,University of São Paulo, 6Health Sciences Department, Ribeirao Preto Medical School,University of Sao Paulo, 7Faculty of Pharmaceutical Sciences,University of São Paulo, 8Department of Genetics, Ribeirão Preto Medical School,University of São Paulo, 9Department of Clinical, Bromatological and Toxicological Analysis, Faculty of Pharmaceutical Sciences of Ribeirao Preto,University of São Paulo
Capítulos
- 00:03Introduction
- 00:43Day 1: Bisulfite Treatment
- 01:21Day 2: Amplification – Methylation Assay, Manual Protocol
- 02:52Day 3: Hybridization – Methylation Assay, Manual Protocol
- 06:39Day 4: Staining – Methylation Assay, Manual Protocol
- 10:48Representative Results
- 14:34Conclusion
Il presente studio descrive il flusso di lavoro per gestire i dati di metilazione del DNA ottenuti dalle tecnologie microarray. Il protocollo illustra i passaggi dalla preparazione del campione all'analisi dei dati. Tutte le procedure sono descritte in dettaglio e il video mostra i passaggi significativi.
