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Basophil Activation Test for Allergy Diagnosis
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JoVE Journal Medicina
Basophil Activation Test for Allergy Diagnosis

Basophil Activation Test for Allergy Diagnosis

DOI:
10.3791/62600-v

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07:22 min

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May 31, 2021

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, , ,
1Allergy Unit,Hospital Regional Universitario de Málaga, 2Allergy Research Group,Instituto de Investigación Biomédica de Málaga-IBIMA, 3Universidad de Málaga, Departamento de Biología celular, Genética y Fisiología,Universidad de Málaga, 4Departamento de Medicina,Universidad de Málaga, 5Nanostructures for Diagnosing and Treatment of Allergic Diseases Laboratory,Andalusian Center for Nanomedicine and Biotechnology-BIONAND

Capítulos

  • 00:05Introduction
  • 01:33Sample and Staining Mix Preparation
  • 02:57Blood Stimulation
  • 03:52Erythrocytes Lysing
  • 04:46Flow Cytometry Acquisition
  • 05:35Results: Basophil Activation Test
  • 06:39Conclusion

Summary

Tadução automática

Tadução automática

The basophil activation test is a complementary in vitro diagnostic test for the evaluation of IgE-mediated allergic reactions based on the detection of basophil activation in the presence of a specific stimulus through the measure of activation markers by flow cytometry.

Tags

Basophil Activation TestAllergy DiagnosisIgE-mediated Allergic ReactionsFlow CytometryActivation MarkersDrug AllergyAllergenPositive ControlFMLPAnti-IgEStaining MixCCR3-APCCD203c-PECD63-FITCPBS-T

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