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使用生物发光成像对小鼠尿路感染及其治疗的纵向随访

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Longitudinal Follow-Up of Urinary Tract Infections and Their Treatment in Mice using Bioluminescence Imaging

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使用生物发光成像对小鼠尿路感染及其治疗的纵向随访

DOI:

10.3791/62614-v

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07:39 min

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June 14, 2021

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Noémie Luyts, Greetje Vande Velde, Matthias Vanneste, Helene De Bruyn, Annelies Janssens, Natalie Verstraeten, Thomas Voets, Wouter Everaerts

¹Laboratory of Ion Channel Research (LICR), VIB-KU Leuven Center for Brain & Disease Research, Leuven, Belgium & Department of Cellular and Molecular Medicine,KU Leuven, ²Biomedical MRI, Department of Imaging & Pathology,KU Leuven, ³Belgium & KU Leuven Centre of Microbial and Plant Genetics,VIB-KU Leuven Center for Microbiology, Leuven, ⁴Laboratory of Experimental Urology, Department of Development and Regeneration,KU Leuven

Capítulos

00:04Introduction
00:57Culturing Bacteria
02:36Inoculation of the Animals
04:04Bioluminescence Imaging
05:49Results: The Analysis of BLI Correlation with CFU at the Time of Instillation and Longitudinal Follow-up During Treatment
07:02Conclusion

Summary

Tadução automática

本手稿描述了使用 勒克斯 操纵子膀胱内施用泌尿道致病细菌以诱导小鼠尿路感染，以及随后使用生物发光成像对细菌负荷进行 纵向体内 分析。

Tags

Keywords: Bioluminescence Imaging Urinary Tract Infections UTI Longitudinal Follow-up Bacterial Culture Inoculum Preparation Anesthesia Intravesical Instillation

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