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Expansion and Enrichment of Gamma-Delta (γδ) T Cells from Apheresed Human Product
JoVE Journal
Pesquisa do Câncer
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JoVE Journal Pesquisa do Câncer
Expansion and Enrichment of Gamma-Delta (γδ) T Cells from Apheresed Human Product

Expansion and Enrichment of Gamma-Delta (γδ) T Cells from Apheresed Human Product

DOI:
10.3791/62622-v

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11:51 min

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September 22, 2021

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, , , , ,
1Cell Therapy Facility,Moffitt Cancer Center and Research Institute, 2Department of Immunology,Moffitt Cancer Center and Research Institute

Capítulos

  • 00:04Introduction
  • 01:29Lymphocyte Isolation and Alpha-beta (αβ) T Cell Depletion
  • 06:16Co-culture with Artificial Antigen-Presenting Cells (aAPCs) and Cell Harvest
  • 09:22Results: Characterization and Optimization of the γδ T Cell Expansion Process
  • 11:21Conclusion

Summary

Tadução automática

Tadução automática

Presented is a protocol for the expansion of Gamma-Delta (γδ) T cell drug product. Lymphocytes are isolated by elutriation and γδ-enriched with zoledronic acid and interleukin-2. Alpha-beta T cells are depleted using a clinical-grade magnetic separation device. The γδ cells are co-cultured with K562-derived, artificial antigen-presenting cells and expanded.

Tags

Keywords: Gamma-delta T CellsCytotoxic ActivityCancerAcute Myeloid LeukemiaExpansionEnrichmentApheresisCell TherapyElutriationCell CultureBioreactorZoledronic AcidIL-2Flow Cytometry

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