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用蛋白毒性应激器治疗大 肠杆菌 后蛋白质聚合物的提取和可视化
JoVE Journal
Bioquímica
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JoVE Journal Bioquímica
Extraction and Visualization of Protein Aggregates after Treatment of Escherichia coli with a Proteotoxic Stressor
DOI:

07:59 min

June 29, 2021

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Capítulos

  • 00:05Introduction
  • 00:53Stress Treatment of E. coli Strains MG1655 and CFT073
  • 02:12Collecting Bacterial Cell Samples
  • 02:43Extracting the Insoluble Protein Aggregates
  • 04:19Soluble Protein Sample Preparation
  • 05:30Separation and Visualization of Extracted Protein Aggregates Using SDS-PAGE
  • 06:49Results: Visualization of Protein Aggregates After Treatment of Escherichia coli with a Proteotoxic Stressor
  • 07:32Conclusion

Summary

Tadução automática

本协议描述了用蛋白质毒性抗菌素治疗后从 大肠杆 菌中提取和可溶性蛋白质的提取和可视化。遵循此程序,可以对不同细菌菌株和/或治疗之间的 体内 蛋白质聚合形成进行定性比较。

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