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En effektiv metode til rettet Hepatocyte-Lignende Cell Induktion fra Human Embryonale stamceller
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
An Efficient Method for Directed Hepatocyte-Like Cell Induction from Human Embryonic Stem Cells

En effektiv metode til rettet Hepatocyte-Lignende Cell Induktion fra Human Embryonale stamceller

DOI:
10.3791/62654-v

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08:05 min

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May 06, 2021

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, , , ,
1Stem Cell Research Center,Shantou University Medical College, 2The Center for Reproductive Medicine,Shantou University Medical College, 3Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology,Shantou University Medical College

Capítulos

  • 00:04Introduction
  • 00:45Stem Cell Maintenance
  • 02:28Stem Cell Passage and Differentiation and Definitive Endoderm Formation
  • 03:46Differentiation of Hepatic Progenitor Cells and Hepatocyte Differentiation
  • 05:12Results: Directed Hepatocyte-like Cell Induction from Human Embryonic Stem Cells
  • 07:14Conclusion

Summary

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Dette manuskript beskriver en detaljeret protokol for differentiering af humane embryonale stamceller (hESC'er) til funktionelle hepatocytlignende celler (HLC' er) ved løbende at supplere Activin A og CHIR99021 under hESC-differentiering til endelig endoderm (DE).

Tags

Keywords: Human Embryonic Stem CellsHepatocyte-like CellsDifferentiationDefinitive EndodermFOXA2SOX17GATA4CXCR4FOXA1MatrigelMTESRActivin ACHIR99021Cell CultureDisease ModelingDrug Screening

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