/

/

利用时间分辨蛋白诱导的荧光增强来鉴定稳定的局部构象，一次一α-突触核蛋白单体

JoVE Journal
Bioquímica

É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.

JoVE Journal Bioquímica

Utilizing Time-Resolved Protein-Induced Fluorescence Enhancement to Identify Stable Local Conformations One α-Synuclein Monomer at a Time

Please note that all translations are automatically generated. Click here for the English version.

利用时间分辨蛋白诱导的荧光增强来鉴定稳定的局部构象，一次一α-突触核蛋白单体

DOI:

10.3791/62655-v

•

07:56 min

•

May 30, 2021

•

Sofia Zaer, Eitan Lerner²

¹Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, Faculty of Mathematics & Science, The Edmond J. Safra Campus,The Hebrew University of Jerusalem, ²The Center for Nanoscience and Nanotechnology,The Hebrew University of Jerusalem

Capítulos

00:05Introduction
00:48Single-Molecule Protein-Induced Fluorescence Enhancement (smPIFE) Sample Preparation and Data Acquisition
02:19smPIFE Burst Analysis
05:07Results: Estimation of Mean Fluorescence Lifetime and Burst Recurrence Analysis
07:12Conclusion

Summary

Tadução automática

时间分辨单分子蛋白诱导的荧光增强是一种有用的荧光光谱接近传感器，对蛋白质的局部结构变化敏感。在这里，我们展示了它可用于揭示α-突触核蛋白中稳定的局部构象，当使用较长距离的FRET标尺测量时，这也称为球状非结构化和不稳定。

Tags

Keywords: Time-resolved Protein-induced Fluorescence Enhancement Single-molecule Alpha-synuclein Structural Subpopulations Local Conformations Sulfo-Cy3 BSA Water Immersion Objective Laser Focus Photon Detection FRETBursts Photon-HDF5

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

利用乙烯释放化合物，2-氯乙基膦酸，作为一种工具来研究细菌乙烯反应

时间分辨电喷雾电离氢氘交换质谱用于研究蛋白质结构与动态

利用综合沉淀富集系统识别目标蛋白的内源性后向转化剖面

利用相扑融合策略生成国产、未加标签的杭丁顿蛋白 Exon1 单体和纤维

操纵活细胞构建稳定的3D 蜂窝组件, 无需人工支架

细胞细胞接触物蛋白质-蛋白质相互作用的荧光波动光谱测定

利用体外荧光共振能量转移研究毫秒时间尺度蛋白质配合物的动力学

识别影响培养哺乳动物细胞在低到中度通量下细胞生长和生存的化合物的策略

快速网格准备时间解决低温电子显微镜

时间分辨Förster共振能量转移测定法，用于测量人细胞中的内源性磷酸化STAT蛋白

Read Article