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Capítulos
- 00:04Introduction
- 00:47Cell Treatment
- 01:56Scanning Electron Microscopy Fixation
- 02:48Critical Point Drying
- 05:16Imaging and Quantification
- 05:58Results: Visualization and Quantification of Membrane Ruffle Formation
- 07:34Conclusion
Macropinocytosis is a highly conserved endocytic process initiated by the formation of F-actin-rich sheet-like membrane projections, also known as membrane ruffles. Increased rate of macropinocytotic solute internalization has been implicated in various pathological conditions. This protocol presents a method to quantify membrane ruffle formation in vitro using scanning electron microscopy.
