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Single Molecule Fluorescence Energy Transfer Study of Ribosome Protein Synthesis
JoVE Journal
Bioquímica
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JoVE Journal Bioquímica
Single Molecule Fluorescence Energy Transfer Study of Ribosome Protein Synthesis
DOI:

08:07 min

July 06, 2021

Capítulos

  • 00:04Introduction
  • 00:52Preparation of the Ribosome Complexes
  • 01:43Preparation of Sample Slides
  • 03:13Single-Molecule FRET Imaging
  • 06:24Results: Single-Molecule Fluorescence Energy Transfer of Ribosome Protein Synthesis
  • 07:16Conclusion

Summary

Tadução automática

Single molecule fluorescence energy transfer is a method that tracks the tRNA dynamics during ribosomal protein synthesis. By tracking individual ribosomes, inhomogeneous populations are identified, which shed light on mechanisms. This method can be used to track biological conformational changes in general to reveal dynamic-function relationships in many other complexed biosystems. Single molecule methods can observe non-rate limiting steps and low-populated key intermediates, which are not accessible by conventional ensemble methods due to the average effect.

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