Journal
/
/
分离子宫先天淋巴细胞,用于流式细胞术分析
JoVE Journal
Imunologia e Infecção
This content is Free Access.
JoVE Journal Imunologia e Infecção
Isolation of Uterine Innate Lymphoid Cells for Analysis by Flow Cytometry
DOI:

09:02 min

October 14, 2021

, , , , ,

Capítulos

  • 00:04Introduction
  • 00:55Mechanical and Enzymatic Digestion of the Uterus
  • 02:35Processing of the Uterus into a Single Cell Suspension
  • 05:22Red Blood Cell Lysis
  • 06:13Results: FACS Analysis of Innate Lymphoid Cells (ILCs)
  • 08:06Conclusion

Summary

Tadução automática

这是一种从怀孕和非怀孕小鼠中分离子宫淋巴细胞的方法。该方法可用于多种下游应用,如FACS表型分析,细胞分选,功能测定,RNA-seq和蛋白质组学。这里的方案演示了如何通过流式细胞术对1组子宫先天淋巴细胞进行表型分析。

Vídeos Relacionados

Read Article