Journal
/
Biologia
/
Determining the Toxicity of UV Radiation and Chemicals on Primary and Immortalized Human Corneal Epithelial Cells
JoVE Journal
Biologia
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Biologia
Determining the Toxicity of UV Radiation and Chemicals on Primary and Immortalized Human Corneal Epithelial Cells

Determining the Toxicity of UV Radiation and Chemicals on Primary and Immortalized Human Corneal Epithelial Cells

DOI:
10.3791/62675-v

•

09:31 min

•

July 22, 2021

•

, , , , , ,
1Centre for Ocular Research & Education (CORE), School of Optometry & Vision Science,University of Waterloo, 2Centre for Eye and Vision Research (CEVR)

Capítulos

  • 00:05Introduction
  • 00:56Determination of Cell Size Using Confocal Microscopy
  • 01:59Exposure of Cells to Ultraviolet (UV) Radiation
  • 03:50Exposure of Cells to Chemical Toxins
  • 05:31Examination of Cells Exposed to Various Concentrations of BAK
  • 06:53Results: Toxicity of UV Radiation and Chemicals on Primary and Immortalized Human Corneal Epithelial Cells
  • 08:50Conclusion

Summary

Tadução automática

Tadução automática

This article describes the procedures used to evaluate the toxicity of UV radiation and chemical toxins on a primary and immortalized cell line.

Tags

ToxicityUV RadiationChemicalsPrimary Human Corneal Epithelial CellsImmortalized Human Corneal Epithelial CellsIn Vitro AssayCell ViabilityAnnexin StainingConfocal MicroscopyCytokine Release

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/62675?article_type=v

Primary Human Bronchial Epithelial Cells Grown from Explants
check_url/pt/62675?article_type=v

Quantification of γH2AX Foci in Response to Ionising Radiation
check_url/pt/62675?article_type=v

Analyzing the Function of Small GTPases by Microinjection of Plasmids into Polarized Epithelial Cells
check_url/pt/62675?article_type=v

Ex Vivo Culture of Primary Human Fallopian Tube Epithelial Cells
check_url/pt/62675?article_type=v

Induction and Analysis of Epithelial to Mesenchymal Transition
check_url/pt/62675?article_type=v

Culturing of Human Nasal Epithelial Cells at the Air Liquid Interface
check_url/pt/62675?article_type=v

Quantitation of Endothelial Cell Adhesiveness In Vitro
check_url/pt/62675?article_type=v

Analysis of SCAP N-glycosylation and Trafficking in Human Cells
check_url/pt/62675?article_type=v

Multicellular Human Alveolar Model Composed of Epithelial Cells and Primary Immune Cells for Hazard Assessment
check_url/pt/62675?article_type=v

Delivery of the Cas9/sgRNA Ribonucleoprotein Complex in Immortalized and Primary Cells via Virus-like Particles (“Nanoblades”)

Read Article