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An Optimized O9-1/Hydrogel System for Studying Mechanical Signals in Neural Crest Cells
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
An Optimized O9-1/Hydrogel System for Studying Mechanical Signals in Neural Crest Cells

An Optimized O9-1/Hydrogel System for Studying Mechanical Signals in Neural Crest Cells

DOI:
10.3791/62693-v

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11:02 min

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August 13, 2021

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, , , , , ,
1Department of Pediatrics, McGovern Medical School,The University of Texas Health Science Center at Houston, 2The University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences,The University of Texas Health Science Center at Houston, 3SEM AFM Core in the Houston Methodist Hospital Research Institute

Capítulos

  • 00:05Introduction
  • 00:34Preparation of Aminosilane-Coated and Siliconized Glass Coverslips
  • 03:13Preparation of Hydrogels
  • 06:40Molecular Analysis of Stiffness via Immunofluorescence Staining
  • 08:04Results: Characterization of O9-1 Cells Grown on Control and Modified Hydrogel System
  • 10:31Conclusion

Summary

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Tadução automática

Detailed step-by-step protocols are described here for studying mechanical signals in vitro using multipotent O9-1 neural crest cells and polyacrylamide hydrogels of varying stiffness.

Tags

Keywords: Neural Crest CellsMechanical SignalsChemical SignalsNeural Crest DevelopmentDiseasesGlass Cover SlipsSterilizationSodium HydroxideAminopropyl TriethoxysilaneGlutaraldehydeDichloromethane SilaneHydrogelAcrylamide Gel

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