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秀丽隐杆线虫运动障碍评估 肌萎缩性侧索硬化症模型
JoVE Journal
Neurociência
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JoVE Journal Neurociência
Evaluation of Motor Impairment in C. elegans Models of Amyotrophic Lateral Sclerosis
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秀丽隐杆线虫运动障碍评估 肌萎缩性侧索硬化症模型

DOI:
10.3791/62699-v

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08:27 min

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September 02, 2021

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1Geriatrics Research Education and Clinical Center,Veterans Affairs Puget Sound Health Care System, 2Division of Gerontology and Geriatric Medicine, Department of Medicine,University of Washington

Capítulos

  • 00:04Introduction
  • 00:45Radial Locomotion Assay
  • 02:10Computer-Analyzed Swimming Assay
  • 05:51Data Analysis
  • 06:19Results: Analysis of the Crawling Speeds in Radial Locomotion and Thrashing in the Swimming Assay
  • 07:48Conclusion

Summary

Tadução automática

Tadução automática

该协议描述了两种敏感的检测方法，用于区分肌萎缩性侧索硬化症秀 丽隐杆线虫 模型中的轻度、中度和重度运动障碍，通常适用于运动改变的 秀丽隐杆线虫 菌株。

Tags

Keywords: C. ElegansAmyotrophic Lateral Sclerosis (ALS)Motor ImpairmentRadial Locomotion AssaySwimming AssayMotility PhenotypesMovement QuantificationComputer-based TrackingThrashing Movements

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