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人性化调解员发布分析作为过敏原效力的读出
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Humanized Mediator Release Assay as a Read-Out for Allergen Potency
DOI:

10:22 min

June 29, 2021

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Capítulos

  • 00:04Introduction
  • 01:04Complement Inactivation of Human Sera
  • 02:05Humanized-RBL Cell Culture
  • 04:07Passive Sensitization of Humanized-RBL Cells
  • 04:57Antigen-stimulated Degranulation and Mediator Release
  • 06:19β-hexosaminidase Activity Measurement
  • 07:21Results: Effect of Allergen on β-hexosaminidase Release
  • 09:38Conclusion

Summary

Tadução automática

在这里,我们介绍介质释放分析,使用与人类IgE受体相互感染的鼠血友病白血病细胞系,模拟在1型过敏反应中通常观察到的效应细胞的去粒。该方法以高度敏感、可重复和可定制的方式研究过敏原的生物活性。

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