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用于登德里脊柱研究的串行块面扫描电子显微镜（SBEM）

JoVE Journal
Neurociência
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JoVE Journal Neurociência

Serial Block-Face Scanning Electron Microscopy (SBEM) for the Study of Dendritic Spines

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用于登德里脊柱研究的串行块面扫描电子显微镜（SBEM）

DOI:

10.3791/62712-v

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11:16 min

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October 02, 2021

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Małgorzata A. Śliwińska, Anna Cały, Jędrzej Szymański, Kasia Radwańska

¹Laboratory of Imaging Tissue Structure and Function,Nencki Institute of Experimental Biology of Polish Academy of Sciences, ²Laboratory of Molecular Basis of Behavior,Nencki Institute of Experimental Biology of Polish Academy of Sciences

Capítulos

00:05Introduction
01:56Sample Contrasting
05:29Dehydration and Resin Embedding
08:02Trimming and Mounting
09:12Serial Block‐Face Scanning Electron Microscopy Imaging
09:47Representative Results: Dendritic Spine Analysis Using Serial Block‐Face Scanning Electron Microscopy
10:35Conclusion

Summary

Tadução automática

连续块面扫描电子显微镜（SBEM）应用于图像和分析穆林海马体中的树突状脊柱。

Tags

Keywords: Serial Block-face Scanning Electron Microscopy Dendritic Spines Synapses Neuronal Plasticity Learning And Memory Sample Preparation Heavy Metal Contrasting Electron Microscopy Hippocampus CA1 Region

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