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Hyperactive piggyBac Transposase-mediated Germline Transformation in the Fall Armyworm, Spodoptera frugiperda
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Biologia
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JoVE Journal Biologia
Hyperactive piggyBac Transposase-mediated Germline Transformation in the Fall Armyworm, Spodoptera frugiperda

Hyperactive piggyBac Transposase-mediated Germline Transformation in the Fall Armyworm, Spodoptera frugiperda

DOI:
10.3791/62714-v

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05:20 min

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September 23, 2021

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,
1Department of Entomology, College of Agriculture, Food and Environment,University of Kentucky

Capítulos

  • 00:04Introduction
  • 00:44Microinjection
  • 02:32Fluorescence Screening and Genetic Crossing
  • 03:34Results: Germline Transformation in the Fall Armyworm, Spodoptera frugiperda
  • 04:44Conclusion

Summary

Tadução automática

Tadução automática

Successful germline transformation in the fall armyworm, Spodoptera frugiperda, was achieved using mRNA of hyperactive piggyBac transposase.

Tags

Keywords: Fall ArmywormSpodoptera FrugiperdaPiggyBacTransposaseGermline TransformationEGFPMicroinjectionArtificial DietPupaeNeonate LarvaeFluorescence Microscopy

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