建立区域特异性人多能干细胞衍生星形胶质细胞和神经元模拟ALS的电生理平台

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Neurociência

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JoVE Journal Neurociência

Establishment of an Electrophysiological Platform for Modeling ALS with Regionally-Specific Human Pluripotent Stem Cell-Derived Astrocytes and Neurons

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建立区域特异性人多能干细胞衍生星形胶质细胞和神经元模拟ALS的电生理平台

DOI:

10.3791/62726-v

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11:52 min

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August 26, 2021

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Arens Taga*¹, Christa W. Habela*¹, Alexandra Johns, Shiyu Liu, Mollie O’Brien, Nicholas J. Maragakis

¹Department of Neurology,Johns Hopkins University

Capítulos

00:04Introduction
00:48Maintaining Non-Confluent Human Induced Pluripotent Stem Cells
02:58Diffrentiation of NPC into Motor Neuron
03:46Co-Culturing Motor Neurons and Astrocytes in Multi-Electrode Array Plates
07:42Multi-Electrode Array Recording
08:16Pharmacological Assays on Multi-Electrode Array
09:01Results: Human Pluripotent Stem Cell-Derived Motor Neurons and Astrocytes
11:09Conclusion

Summary

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我们描述了一种区分脊髓人诱导的多能源星形胶质细胞和神经元及其共培养以进行电生理记录的方法。

建立区域特异性人多能干细胞衍生星形胶质细胞和神经元模拟ALS的电生理平台

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