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Imunologia e Infecção
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Mesenchymal Stem Cell Regulation of Macrophage Phagocytosis; Quantitation and Imaging
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Mesenchymal Stem Cell Regulation of Macrophage Phagocytosis; Quantitation and Imaging

Mesenchymal Stem Cell Regulation of Macrophage Phagocytosis; Quantitation and Imaging

DOI:
10.3791/62729-v

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09:10 min

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July 16, 2021

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, , , , ,
1Molloy College

Capítulos

  • 00:04Introduction
  • 00:50Mesenchymal Stem Cells Culture in Experimental Dish
  • 03:01Activation of MΦ (Macrophage Cells) with IFN-γ
  • 03:44MΦ (Macrophage Cells) Isolation and Co-culture with Mesenchymal Stem Cells
  • 04:53Zymosan Preparation and Addition to the Co-cultured Cells
  • 06:09Phagocytosis Assay and Dynamic Imaging
  • 07:57Results: Effect of IFN-γ on Phagocytosis
  • 08:44Conclusion

Summary

Tadução automática

Tadução automática

Presented here is a protocol to quantify and produce dynamic images of mesenchymal stem cell (MSC) mediated regulation of macrophage (MΦ) phagocytosis of non-opsonized yeast (zymosan) particles that are conjugated to a pH-sensitive fluorescent molecule.

Tags

Mesenchymal Stem CellsMacrophage PhagocytosisCell CultureCo-cultureBioparticlesPH-sensitive DyesPhagocytesCell CountingTrypan BlueHemocytometerCell Suspension

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