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自动、高通量检测细菌对宿主细胞的粘附

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Automated, High-Throughput Detection of Bacterial Adherence to Host Cells

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自动、高通量检测细菌对宿主细胞的粘附

DOI:

10.3791/62764-v

•

07:21 min

•

September 17, 2021

•

Jing Yang, Qing-Ming Qin, Erin Van Schaik, James E. Samuel, Paul de Figueiredo²

¹Department of Microbial Pathogenesis and Immunology,Texas A&M Health Science Center, ²College of Veterinary Medicine,Texas A&M University

Capítulos

00:04Introduction
00:48Staining
02:20Bacterial Adherence Assay
04:01Automated Fluorescence Imaging, Processing, and Analysis
05:24Results: Analysis of Host Cell-Adhered Bacteria
06:46Conclusion

Summary

Tadução automática

使用高通量荧光标记成像以及自动统计分析方法，基于表型粘附性检测宿主 - 细菌病原体相互作用，可以快速评估细菌与宿主细胞的潜在相互作用。

Tags

Keywords: Bacterial Adherence High-throughput Detection Host Cells Automated Time-saving Reproducible Multiplicity Of Infection Bacterial Suspension Staining Bacterial Adherence Assay A549 Cells PBS Formaldehyde

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