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Microscopía de fluorescencia para internalización de ATP mediada por macropinocitosis en células tumorales humanas y ratones xenoinjertados con tumores
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1Department of Biological Sciences,Ohio University, 2Molecular and Cellular Biology Program,Ohio University, 3Neuroscience Program,Ohio University, 4Edison Biotechnology Institute,Ohio University, 5Translational Biomedical Sciences Program,Ohio University, 6Honors Tutorial College,Ohio University, 7Department of Biomedical Sciences, Heritage College of Osteopathic Medicine,Ohio University
Capítulos
- 00:05Introduction
- 01:05Tumor Cell Culture and Xenograft Tumor Development
- 02:41HMWFD and NHF-ATP Solution Preparation
- 03:21ATP Internalization in Tumor Xenograft
- 03:53Cryo-embedding
- 04:45Tissue Slide Fixation
- 05:25Fluorescence Microscopy and Image Acquisition
- 07:12Results: Assessment of Nonhydrolyzable Fluorescent ATP Internalization
- 07:44Conclusion
Desarrollamos un método reproducible para visualizar la internalización del trifosfato de adenosina fluorescente no hidrolizable (ATP), un sustituto de ATP, con alta resolución celular. Validamos nuestro método utilizando ensayos independientes in vitro e in vivo: líneas celulares tumorales humanas y ratones inmunodeficientes xenoinjertados con tejido tumoral humano.
