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Biologia do Desenvolvimento

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转变生长因子的分析 - 家族产品分泌到 异种胚胎 的爆炸

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Biologia do Desenvolvimento

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JoVE Journal Biologia do Desenvolvimento

Analysis of Transforming Growth Factor ß Family Cleavage Products Secreted Into the Blastocoele of Xenopus laevis Embryos

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转变生长因子的分析 - 家族产品分泌到异种胚胎的爆炸

DOI:

10.3791/62782-v

•

06:57 min

•

July 21, 2021

•

Hyung-Seok Kim, Jan L. Christian

¹Department of Neurobiology,University of Utah, ²Departments of Neurobiology and Department of Internal Medicine, Division of Hematology and Hematologic Malignancies,University of Utah, School of Medicine

Capítulos

00:04Conclusion
00:50Blastocoele Extraction and Analysis of Tgf β Cleavage Products
04:49Results: Analysis of Cleaved BMP Ligands in Xenopus laevis Blastocoele Fluid
06:08Conclusion

Summary

Tadução automática

当转化生长因子 +家族前体蛋白在 Xenopus laevis 胚胎中异位表达时，它们变暗、裂开并分泌到从晚期到早期胃肠阶段的胚芽。我们描述了一种从气穴中吸气产品的方法，用于免疫腹腔分析。

Tags

Keywords: TGF-beta Xenopus Laevis Embryos Blastocele Fluid Proteolytic Cleavage Aspirate Microinjector Immunoblot Precursor Proteins Cleavage Products

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