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Transient Transduction of the Strobilated Forms of Echinococcus granulosus
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JoVE Journal Genética
Transient Transduction of the Strobilated Forms of Echinococcus granulosus

Transient Transduction of the Strobilated Forms of Echinococcus granulosus

DOI:
10.3791/62783-v

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13:25 min

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September 16, 2022

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, , , , , ,
1Student Research Committee, School of Medicine,Kerman University of Medical Sciences, 2Research Center for Hydatid Disease in Iran,Kerman University of Medical Sciences, 3Department of Parasitology and Mycology, Faculty of Medicine,Kurdistan University of Medical Sciences, 4State Key Laboratory for Zoonotic Diseases, Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine,Jilin University

Capítulos

  • 00:15Introduction
  • 01:34Collecting Hydatid Cysts
  • 03:06Biphasic Cultivation of Protoscoleces of E. granulosus to Obtain Adult Worms
  • 03:59Monophasic Cultivation of Protoscoleces of E. granulosus
  • 04:27Cell Culture for Virus Production and Preparation
  • 05:06Production and Preparation of the Virus with the Third-Generation Lentiviral Vectors
  • 07:49Transient Transduction of Different Stages of E. granulosus with the Virus
  • 09:44Representative Results
  • 11:28Conclusion

Summary

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We describe a rapid transient transduction technique in different developmental stages of Echinococcus granulosus using third-generation lentiviral vectors.

Tags

Keywords: Echinococcus GranulosusCystic EchinococcosisHydatid DiseaseZoonotic Parasitic DiseaseProtoscolecesStrobilated WormsTransient TransductionLentiviral VectorsGene TransferCestode ParasitesGene Evaluation SystemViral Delivery SystemGFP Reporter GeneBiphasic Cultivation

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