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通过流式细胞术进行质量控制痰液分析

JoVE Journal
Medicina

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JoVE Journal Medicina

Quality-Controlled Sputum Analysis by Flow Cytometry

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通过流式细胞术进行质量控制痰液分析

DOI:

10.3791/62785-v

•

07:22 min

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August 09, 2021

•

Marcia Grayson*¹, Shao-Chiang Lai*¹, Lydia H. Bederka, Patricia Araujo, Jamila Sanchez, Xavier T. Reveles, Vivienne I. Rebel², Jennifer Rebeles

¹bioAffinity Technologies, ²Department of Cell Systems & Anatomy,The University of Texas Health Science Center at San Antonio

Capítulos

00:04Introduction
01:00Sputum Dissociation
02:30Antibody and Viability Dye Staining
03:37Fixation with 1% Paraformaldehyde
04:40Flow Cytometer – Data Acquisition
05:33Results: Sputum Analysis by Flow Cytometry
06:59Conclusion

Summary

Tadução automática

该协议描述了一种将痰液解离为单细胞悬浮液的有效方法，以及随后在标准流式细胞术平台上对细胞亚群的表征。

Tags

Keywords: Sputum Analysis Flow Cytometry Quality Control Cell Count COPD Asthma Lung Cancer COVID-19 NAC DTT HBSS Trypan Blue Hemocytometer Compensation Tubes Antibody Staining Dye Staining

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