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Probing Structural and Dynamic Properties of Trafficking Subcellular Nanostructures by Spatiotemporal Fluctuation Spectroscopy
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Probing Structural and Dynamic Properties of Trafficking Subcellular Nanostructures by Spatiotemporal Fluctuation Spectroscopy
DOI:

08:17 min

August 16, 2021

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Capítulos

  • 00:04Introduction
  • 00:58Sample Preparation
  • 02:35Data Acquisition
  • 03:52iMSD Calculation
  • 05:54Results: Structural and Dynamic Properties of Lysosomes and Macropinosomes
  • 07:45Conclusion

Summary

Tadução automática

Imaging-derived mean square displacement (iMSD) analysis is applied to macropinosomes to highlight their intrinsic time-evolving nature in terms of structural and dynamic properties. Macropinosomes are then compared to insulin secretory granules (ISGs) as a reference for subcellular structures with time-invariant average structural/dynamic properties.

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