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Automated Microbial Cultivation and Adaptive Evolution using Microbial Microdroplet Culture System (MMC)
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1Department of Chemical Engineering, Institute of Biochemical Engineering,Tsinghua University, 2Key Laboratory of Industrial Biocatalysis, Ministry of Education,Tsinghua University, 3Luoyang TMAXTREE Biotechnology Co., Ltd., 4Biochemical Engineering Research Group, School of Chemical Engineering and Technology,Xi’an Jiaotong University, 5Center for Synthetic & Systems Biology,Tsinghua University, 6School of Life Science and Technology,Tokyo Institute of Technology
Capítulos
- 00:04Introduction
- 00:51Preparations for the Experiment
- 01:48Growth Curve Measurement in Microbial Microdroplet Culture System (MMC)
- 04:25Adaptive Evolution in Microbial Microdroplet Culture System (MMC)
- 06:41Results: The Adaptive Evolution of the Methanol-Essential Escherichia coli (MeSV2.2) in MMC
- 07:36Conclusion
This protocol describes how to use the Microbial Microdroplet Culture system (MMC) to conduct automated microbial cultivation and adaptive evolution. MMC can cultivate and sub-cultivate microorganisms automatically and continuously and monitor online their growth with relatively high throughput and good parallelization, reducing labor and reagent consumption.
Tags
Automated Microbial CultivationAdaptive EvolutionMicrobial Microdroplet Culture System (MMC)Low-costOperation-friendlyReliable ResultsSimple OperationsMicrobiology ResearchGrowth Curve MeasurementAdaptive Laboratory EvolutionSingle Factor Multi-level AnalysisMicrofluidic ChipEscherichia Coli MG1655Cultivation TemperaturePhotoelectric SignalMMC OilInitial Bacteria Solution
