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Deep and Spatially Controlled Volume Ablations using a Two-Photon Microscope in the Zebrafish Gastrula
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Capítulos
- 00:05Introduction
- 00:38Preparation of the Two-photon Microscope
- 02:19Mounting the Embryo
- 03:46Locating the Embryo and Pre-Ablation Imaging
- 04:53Target Location, Laser Ablation and Post-ablation Verification
- 07:20Results: Analysis of the Zebrafish Embryo Before and Post Ablation
- 09:07Conclusion
Embryonic development requires large-scale coordination of cell motion. Two-photon excitation mediated laser ablation allows the spatially controlled 3-dimensional ablation of large groups of deep cells. In addition, this technique can probe the reaction of collectively migrating cells in vivo to perturbations in their mechanical environment.
