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Imunologia e Infecção

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骨髓来源巨噬细胞的分离和体外培养用于NO-氧化还原生物学研究

JoVE Journal
Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Isolation and In vitro Culture of Bone Marrow-Derived Macrophages for the Study of NO-Redox Biology

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骨髓来源巨噬细胞的分离和体外培养用于NO-氧化还原生物学研究

DOI:

10.3791/62834-v

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06:34 min

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May 31, 2022

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Marina Diotallevi*^1,2, Thomas Nicol*^1,2, Faseeha Ayaz², Jade Bailey², Andrew Shaw², Eileen McNeill², Ben Davies², Keith M. Channon², Mark J. Crabtree²

¹BHF Centre of Research Excellence, Division of Cardiovascular Medicine, Radcliffe Department of Medicine, John Radcliffe Hospital,University of Oxford, ²Wellcome Centre for Human Genetics,University of Oxford

Capítulos

00:04Introduction
00:53Isolation of Bone Marrow Cells
02:14Selection, Differentiation, and Stimulation of BMDMs
04:24Results: Comparison of Nitrite and Tetrahydrobiopterin Levels in Media Supplemented with LCM or Recombinant GM-CSF and M-CSF
05:54Conclusion

Summary

Tadução automática

该方案已被建立用于培养四氢生物蝶呤（BH4）-和诱导的一氧化氮合酶（iNOS）缺陷原代鼠巨噬细胞，以研究NO-氧化还原生物学。该研究的重点是减少传统分离和培养方法中发现的BH4和其他伪影的潜在污染，这些伪影可能会混淆实验结果和对结果的解释。

Tags

Keywords: Bone Marrow-derived Macrophages INOS BH4 Nitric Oxide Redox Biology In Vitro Culture Cell Isolation Cell Disaggregation Cell Freezing DMEM/F-12 Media

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