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Direct Stochastic Optical Reconstruction Microscopy of Extracellular Vesicles in Three Dimensions
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Biologia
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JoVE Journal Biologia
Direct Stochastic Optical Reconstruction Microscopy of Extracellular Vesicles in Three Dimensions

Direct Stochastic Optical Reconstruction Microscopy of Extracellular Vesicles in Three Dimensions

DOI:
10.3791/62845-v

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09:36 min

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August 26, 2021

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, ,
1Department of Microbiology and Immunology, Lineberger Comprehensive Cancer Center,The University of North Carolina at Chapel Hill School of Medicine

Capítulos

  • 00:04Introduction
  • 00:56Fixation and Preparation
  • 02:21Direct Stochastic Optical Reconstruction Microscopy Calibration
  • 04:56Visualization of EV in Three Dimensions
  • 06:16Post-Acquisition Modification and EV Tracing
  • 07:13Results: Direct Stochastic Optical Reconstruction Microscopy of Extracellular Vesicles
  • 09:00Conclusion

Summary

Tadução automática

Tadução automática

Direct stochastic optical reconstruction microscopy (dSTORM) is used to bypass the typical diffraction limit of light microscopy and to view exosomes at the nanometer scale. It can be employed in both two and three dimensions to characterize exosomes.

Tags

Extracellular VesiclesDSTORMSuper-resolution MicroscopyThree-dimensional VisualizationVirus ParticlesDisease BiomarkersAffinity PurificationParaformaldehyde FixationImaging BufferCalibration Beads

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