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三维细胞外囊泡的直接随机光学重建显微镜

JoVE Journal
Biologia

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JoVE Journal Biologia

Direct Stochastic Optical Reconstruction Microscopy of Extracellular Vesicles in Three Dimensions

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三维细胞外囊泡的直接随机光学重建显微镜

DOI:

10.3791/62845-v

•

09:36 min

•

August 26, 2021

•

Meredith G. Chambers, Ryan P. McNamara, Dirk P. Dittmer

¹Department of Microbiology and Immunology, Lineberger Comprehensive Cancer Center,The University of North Carolina at Chapel Hill School of Medicine

Capítulos

00:04Introduction
00:56Fixation and Preparation
02:21Direct Stochastic Optical Reconstruction Microscopy Calibration
04:56Visualization of EV in Three Dimensions
06:16Post-Acquisition Modification and EV Tracing
07:13Results: Direct Stochastic Optical Reconstruction Microscopy of Extracellular Vesicles
09:00Conclusion

Summary

Tadução automática

直接随机光学重建显微镜（dSTORM）用于绕过光学显微镜的典型衍射极限，并在纳米尺度上观察外泌体。它可以在二维和三维空间中用于表征外泌体。

Tags

Keywords: Extracellular Vesicles DSTORM Super-resolution Microscopy Three-dimensional Visualization Virus Particles Disease Biomarkers Affinity Purification Paraformaldehyde Fixation Imaging Buffer Calibration Beads

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