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Absolute Quantitation of Inositol Pyrophosphates by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry
JoVE Journal
Bioquímica
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JoVE Journal Bioquímica
Absolute Quantitation of Inositol Pyrophosphates by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry

Absolute Quantitation of Inositol Pyrophosphates by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry

DOI:
10.3791/62847-v

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09:22 min

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August 13, 2021

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, , ,
1Institute of Organic Chemistry,University of Freiburg, 2Medical Research Council, Laboratory for Molecular Cell Biology,University College London

Capítulos

  • 00:04Introduction
  • 00:45Setting Up the Capillary Electrophoresis Electrospray Ionization Mass Spectrometry (CE-ESI-MS) System
  • 01:38Preparation of Buffer, Capillary, and CE-MS System
  • 03:28Performing the CE-ESI-MS Runs
  • 05:10Data Analysis
  • 06:50Results: Quantitation of Inositol Pyrophosphates from Mammalian Cell Extracts by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry
  • 08:35Conclusion

Summary

Tadução automática

Tadução automática

A procedure for capillary electrophoresis electrospray ionization mass spectrometry for the absolute quantitation of inositol pyrophosphates from mammalian cell extracts is described.

Tags

Inositol PyrophosphatesCapillary ElectrophoresisElectrospray IonizationMass SpectrometryCE-ESI-MSInositol Phosphate MetabolismQuantitationBiosynthetic PathwayCE Running BufferSheath LiquidCapillary ColumnESI SprayerInositol Phosphate Standards

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