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Fremstilling af multifunktionelle silkebaserede mikrokapsler fyldt med DNA-plasmider, der koder for RNA-aptamerere og ribotekser
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Biologia
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JoVE Journal Biologia
Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches

Fremstilling af multifunktionelle silkebaserede mikrokapsler fyldt med DNA-plasmider, der koder for RNA-aptamerere og ribotekser

DOI:
10.3791/62854-v

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10:07 min

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October 08, 2021

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, , ,
1UES Inc., 2711th Human Performance Wing, Airman Systems Directorate, Air Force Research Laboratory,Wright-Patterson AFB, 3Materials and Manufacturing Directorate,Wright-Patterson AFB

Capítulos

  • 00:04Introduction
  • 00:56Perform Layer‐by‐Layer Deposition of a Prime Layer, DNA Plasmids, and Silk Layers
  • 04:44Dissolution of SiO2Cores and Imaging of Silk Fibroin Microcapsules
  • 05:42Estimation of the Permeability of Hollow Microcapsules Using Molecular Weight Cut‐Off (MWCO) Method
  • 06:42In Vitro Activation of Synthetic Theophylline Riboswitch in Silk Microcapsules
  • 07:28Results: Microscopy Studies, Membrane Permeability, and Gene Expression Kinetics of Silk Fibroin Microcapsules
  • 09:25Conclusion

Summary

Tadução automática

Tadução automática

Protokollen beskriver dannelsen af robuste og biokompatible DNA-belastede mikrokapsler som multipleksede de vitro-biosensorer, der er i stand til at spore flere ligander.

Tags

Multifunctional Silk-based MicrocapsulesDNA PlasmidsRNA AptamersRiboswitchesBiocompatible MicrocapsulesDNA ImmobilizationBiosensorsBiological ProcessesSignaling MoleculesGene ActivationPolyethyleneimineSilica MicroparticlesDNA PlasmidsTheophylline RiboswitchBroccoli AptamerSilk Fibroin

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