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Isolation and Selection of Entomopathogenic Fungi from Soil Samples and Evaluation of Fungal Virulence against Insect Pests
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Biologia
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JoVE Journal Biologia
Isolation and Selection of Entomopathogenic Fungi from Soil Samples and Evaluation of Fungal Virulence against Insect Pests

Isolation and Selection of Entomopathogenic Fungi from Soil Samples and Evaluation of Fungal Virulence against Insect Pests

DOI:
10.3791/62882-v

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09:42 min

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September 28, 2021

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, , , , , ,
1Department of Entomology,National Chung Hsing University, 2Department of Agricultural biology,Jeonbuk National University, 3Department of Agricultural Convergence Technology,Jeonbuk National University

Capítulos

  • 00:04Introduction
  • 01:04Isolation and Selection of Potential Entomopathogenic Fungi (EPF)
  • 02:271st Pathogenicity Screen and 2nd Virulence Test of Fungal Isolates
  • 03:42Molecular and Morphological Identification of Potential Entomopathogenic Fungi (EPF)
  • 05:23Investigation of Conidial Productivity and Thermotolerance
  • 07:07Effective Conidia Number (ECN) Ranking
  • 07:31Results: Thermotolerance and Conidial Production Data into the ECN Formula and PCA Analysis
  • 08:40Conclusion

Summary

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Here we present a protocol based on the mealworm (Tenebrio molitor)-bait system that was used for isolating and selecting entomopathogenic fungi (EPF) from soil samples. An effective conidia number (ECN) formula is used to select high stress tolerant EPF based on physiological characteristics for pest microbial control in the field.

Tags

Keywords: Entomopathogenic FungiSoil SamplesInsect PestsIsolationVirulencePathogenicity ScreeningTenebrio MolitorSabouraud Dextrose AgarThermotoleranceConidia Production

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