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Fluorescensaktivert cellesortering-radioligog behandlet vev (FACS-RTT) for å bestemme cellulær opprinnelse av radioaktivt signal
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1Division of Adult Psychiatry, Department of Psychiatry,University Hospitals of Geneva, 2Department of Psychiatry,University of Geneva, 3Division of Nuclear medicine and Molecular Imaging,University Hospitals of Geneva, 4Division of Radiation Oncology, Department of Oncology,University Hospitals of Geneva, 5Department of Brain Sciences, Faculty of Medicine,Imperial College London
Capítulos
- 00:04Introduction
- 00:38SPECT Camera Preparation and Calibration
- 01:47[125I]CLINDE Radiotracer Synthesis
- 04:27SPECT Acquisition
- 05:15Scan Reconstruction
- 05:57Rat Brain Extraction and Cell Isolation
- 09:46Results: FACS-RTT to Determine the Cellular Origin of Radioactive Signal
- 11:27Conclusion
Fluorescensaktivert cellesortering-radioligog behandlet vev (FACS-RTT) er et kraftig verktøy for å studere rollen til 18 kDa translokatorprotein eller Serotonin 5HT2A-reseptoruttrykk i Alzheimers sykdom i cellulær skala. Denne protokollen beskriver ex-vivo-applikasjonen av FACS-RTT i TgF344-AD-rottemodellen.
