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用于疫苗接种目的的mRNA负载的聚（β-氨基酯）纳米颗粒的合成和表征
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Bioengenharia
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JoVE Journal Bioengenharia
Synthesis and Characterization of mRNA-Loaded Poly(Beta Aminoesters) Nanoparticles for Vaccination Purposes
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用于疫苗接种目的的mRNA负载的聚（β-氨基酯）纳米颗粒的合成和表征

DOI:
10.3791/62889-v

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08:27 min

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August 13, 2021

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, , , , , , ,
1Grup d’Enginyeria de Materials (Gemat), Institut Químic de Sarrià (IQS),Universitat Ramon Llull (URL)

Capítulos

  • 00:04Introduction
  • 01:00Polyplexes Formation, Lyophilization, and Resuspension
  • 02:44In Vitro Characterization
  • 04:34In Vitro Functionality Tests: Capacity to Activate Model Immune Cells by Using Ovalbumin as Antigenic Model mRNA
  • 06:24Results: Nanoparticle Synthesis and Physicochemical Characterization, mRNA Encapsulation Efficiency, and In Vitro Results
  • 07:45Conclusion

Summary

Tadução automática

Tadução automática

在这里，提出了一种基于聚（β-氨基酯）聚合物的mRNA纳米颗粒的简单方案，易于通过改变封装的mRNA进行定制。还描述了合成聚合物，纳米颗粒及其 体外 基本表征的工作流程。还增加了关于免疫接种的概念验证。

Tags

MRNANanoparticlesPoly(beta Aminoesters)VaccinationNucleic AcidsPolymeric NanoparticlesLyophilizationPolyplexesTransfectionHela CellsFluorescence Microscopy

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