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Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing
 

Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing

Article DOI: 10.3791/62901-v • 06:00 min • September 20th, 2021
September 20th, 2021 •
*1,2, *1,2, 3, 4, 3, 3, 1,2
1Department of Nephrology and Medical Intensive Care Medicine, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, 2Molecular and Translational Kidney Research, Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), 3Berlin Institute of Medical Systems Biology (BIMSB), Systems Biology of Gene Regulatory Elements, Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), 4Berlin Institute of Medical Systems Biology (BIMSB), Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC)
* These authors contributed equally

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Here, we present a protocol to isolate high-quality nuclei from frozen mouse kidneys that improve the representation of medullary kidney cell types and avoids the gene expression artifacts from enzymatic tissue dissociation.

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Nuclei Isolation Single-nucleus RNA-sequencing Kidney Tissue Cortex Medulla RNA Stabilization Nuclei Lysis Buffer Tissue Homogenization Cell Lysis Cell Straining
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